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Montreal is one of my top locations to visit, I have wanted to explore it for years and need to make it a prioirty to get there soon!

Patti

Marie-Eve, we used your blog to plan our visit to Montreal last month. We stayed in Mile End, went on a food tour, and generally had a great time. Thanks for all your useful information. (Next update, how about mentioning the Guilliaume bakery? It was a frequent stop for us…. amazing baking!)

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Montreal is so beautiful! I spent a couple of weeks there but there was so much to do, it broke my heart to leave without doing it and seeing it all!

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pretty streets:)

Valerie B.

The Plateau is my fave neighbourhood! It is so lively. Every thing is within walking distance…i love that so much that I live in the plateau!

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What a fabulous place! Thanks for sharing your trip experience and wonderful tips. Your photos are gorgeous and show so much culture. Continue to be an unstoppable traveler! Cheers, Rhonda

Whoa ! I didn’t know anything about Montreal, but now, I really want to go there ! We are currently travelling in south America from South to North, maybe we’ll be able to get to Canada and visit this wonderful City !

Emma Halvey

Great post! Visiting Montreal in November, will definitely keep these tips in mind

John phine

I utilized your blog to design our visit to Montreal a month ago. We remained in Mile End, went on a sustenance visit, and for the most part had an awesome time. Much obliged for all your helpful data

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That so beautiful and artist city. I really love go there once!

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So awesome city. Thanks for describing The city’s park, Coffe shop, street. Future these information are very helpful for me.

budget jan

We were there in 2010 and I can see from your gorgeous photos that Plateau has only got better. I certainly want to stay there next time we’re in Montreal.

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Hi Marie!

This entry got me inspired! What’s not to love about Plateau Mont-Royal? My favorite things are the murals, they make the perfect backdrop for Instagram photos that the artsy crowd will like.

And because I support local artisans, I am quite excited to check out several shops that sell unique pieces that I can add to my collection!

Marie-Eve

Hope you’ll have a great trip!

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[…] Send me your Montreal recommendations! I’m going to the Canadian city for a quick vacation later this month and I want your suggestions! I’m so excited since the last time I went to the city was when I was ten. It’s been on my list for years and I can’t wait to explore the city. Any coffee shop, bookstores, boutiques, restaurants, bars, live music that you love would be amazing. I can’t wait to pretend I’m in Europe for a few days. (Photo via To Europe Beyond.) […]

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1. Beyonce

Bow down to the Queen. Beyonce slayed the competition as Womens Pirasa LowTop Sneakers Aldo PrjyqDAsq
with a mammoth $62.1 million -- $20 million more than the No. 2 act. The bulk of that payday stemmed from her Formation World Tour, in support of her sixth album, ,an all-stadium trek that boasted DJ Khaled and Rae Sremmurd as opening acts, not to mention Kendrick Lamar and husband JAY-Z as surprise guests at the MetLife Stadium finale in East Rutherford, N.J. That tour sold 1.2 million tickets and grossed an industry-leading $161 million across 32 North American dates, but Bey didn’t stop there. Finishing in the top 15 in every category we measured, she was one of only six artists to earn more than $1 million in each category and ranked sixth in total artist royalties ($6.2 million). And while may have lost the Grammy for album of the year, it earned 2.2 million equivalent album units in 2016, according to Nielsen Music (and added 260,000 more this year). LAST YEAR: N/A

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2. Guns N' Roses

What began with a secret show at the tiny Troubadour in Los Angeles last April became GNR’s highest-grossing tour to date. Despite Axl Rose’s doubts, the tour is still ongoing, accounting for most of the group’s $42 million in earnings in 2016. LAST YEAR: N/A

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3. Bruce Springsteen

The Boss had 2016’s second-highest-grossing tour, yielding over $40 million for himself. He banked $1.4 million in recording royalties, helped by sales of both his catalog and a late-2015 box set celebrating the 35th anniversary of LAST YEAR: N/A

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4. Drake

After dominating the charts last year, Drake led all acts in on-demand audio and video streams (6.8 billion), plus royalties with $23.7 million. Adding nearly $14 million for his summer tour with Future, Drake danced away with over $37 million in 2016. LAST YEAR: 32

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5. Adele

The “Rolling in the Deep” singer is rolling in the dough. Adele’s tour was 2016’s fourth-highest grossing, and she moved 2.2 million albums in the United States, more than any other artist except Prince; she also clocked nearly 1.3 million radio spins. LAST YEAR: 9

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6. Coldplay

Sparked by the band’s performance at the Super Bowl 50 Halftime Show, Coldplay ranked No. 5 at the box office and raked in $26 million on the road in 2016, selling 323,000 copies of its album in the United States in the process. LAST YEAR: N/A

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7. Justin Bieber

Even in light of Bieber’s impressive touring income, his streaming power shines, as he racked up nearly as many on-demand video streams (1.2 billion) as audio streams (1.5 billion). He was also No.3 in terrestrial radio spins. LAST YEAR: N/A

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8. Luke Bryan

Bryan can thank his fan club, the Nut House, for fueling ticket sales that generated the country star’s take-home pay of $23 million. The ninth-most-played act on the airwaves (with 1.1 million spins) added $4.1 million in artist and songwriter royalties. LAST YEAR: 7

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9. Kanye West

A no-show on 2015’s list, West ranks as the second-highest-grossing rapper behind Drake, thanks in part to his $15 million take from the blockbuster Saint Pablo Tour ($45 million). He’s second only to Drake in audio on-demand streams, too (2 billion). LAST YEAR: N/A

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10. Kenny Chesney

Chesney’s haul included $23.5 million in take-home pay from $69 million in ticket sales, making him the seventh-largest touring act in the United States in 2016. He was also No.15 in radio airplay (886,000 spins) and tallied nearly $2 million from recording. LAST YEAR: 2

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11. Billy Joel

The Piano Man played his way to $23.6 million in 2016, primarily on the strength of his New York Madison Square Garden residency. Just as impressive: $1.2 million in artist and publishing royalties, plus a respectable 192 million audio on-demand streams. LAST YEAR: 4

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12. Rihanna

Rihanna put in plenty of work on the road in 2016, but her streaming success was huge, too: She came in third in on-demand audio (2 billion) and first in on-demand video (1.3 billion), and earned $10.1 million in master recording royalties, ranking fourth. LAST YEAR: N/A

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13. Twenty One Pilots

The alt-rockers’ recorded-music and publishing royalties alone (at $15 million) would have ensured them a place in this year’s rankings. For publishing, the “Stressed Out” hitmakers rank No. 1; they were also 2016’s third-most-streamed act. LAST YEAR: N/A

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14. Carrie Underwood

The superstar crisscrossed the country in 2016 on a $52.3 million-grossing tour, snaring the last spot on the top 10 list of live U.S. acts. That translated to $17.8 million for Underwood, whose recorded music netted her another $2.1 million in royalties. LAST YEAR: N/A

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15. Metallica

Metallica had a light touring year, but its (the band’s sixth No. 1 on the Billboard 200) helped yield 2.7 million album and track sales, making the group, which owns its own masters, No. 1 in total royalties ($14.7 million). LAST YEAR: N/A

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16. Paul McCartney

Road warrior Sir Paul pocketed over $16 million from his $48 million-grossing 2016 One on One Tour. And despite no new releases, McCartney, who owns his masters and wrote many of his hits, nabbed $1.3 million in artist and songwriting royalties. LAST YEAR: 33

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17. Future

The first artist with back-to-back Billboard 200 No. 1 debuts in successive weeks, Future earned nearly $14 million as Drake’s Summer Sixteen Tour co-headliner and ranked in the top 10 in on-demand audio (1.5 billion) and video (1.1 billion) streams. LAST YEAR: N/A

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18. Garth Brooks

Brooks was one of the industry's top earners in sales royalties with $7.6 million. His heftyrecording royalties total from sales is no doubt partly attributable not only to the commercial strength of Brooks' back catalog -- which includes a stupefying seven RIAA diamond-certified releases -- but the fact that his music had long been kept off streaming services, until his Amazon Prime deal in October. His touring totaled $25 million, which amounted to $8.2 million in take-home pay. But that estimate is incomplete because Brooks'tour did not report its grosses to Billboard Boxscore. If his tour had reported to Boxscore, he may have very well finished among the top five earners of 2016.LAST YEAR: N/A

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19. Trans-Siberian Orchestra

The perennial Christmas touring titans grossed nearly $45 million at the box office, taking home $15.1 million themselves. And with 2016’srelease, it also earned over $500,000 in album sale royalties alone. LAST YEAR: N/A

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20. Barbra Streisand

Streisand hit No 1. on the Billboard 200 with and generated nearly $900,000 in artist royalties. But the bulk of the EGOT legend’s revenue came from the stage: $14.9 million from a $44 million box-office total. LAST YEAR: N/A

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21. AC/DC

A touring behemoth, the band netted $27.2 million in 2016 from ticket sales, nabbing a take of $9.2 million. But because the rockers also own their recorded masters, they enjoy much higher royalties than their peers, bringing in another $5.8 million. LAST YEAR: 24

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22. Dixie Chicks

They haven’t released a new album in over a decade, so combined artist and publishing royalties barely cleared half a million. But the Chicks remain a top touring act: 2016’s globe-spanning DCX MMXVI trek earned them $14.2 million in the United States. LAST YEAR: N/A

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23. Florida Georgia Line

Newcomers to the list caused the duo’s ranking to slip, but it still posted stronger numbers across the board than in 2015, including $1.5 million in sales royalties, higher than fellow country powerhouses Luke Bryan and Carrie Underwood. LAST YEAR: 19

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24. Dave Matthews Band

A mainstay on this list thanks to its devoted live following, DMB bettered its 2015 touring total by nearly half a million with its 25th-anniversary summer tour. Though it was a stronger year for live acts, the band still slipped nine spots overall. LAST YEAR: 13

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25. Jason Aldean

The country star’s touring numbers sagged by over $5 million in 2016, thanks to fewer dates on his We Were Here Tour than on 2015’s Burn It Down trek. But his streaming numbers tripled, likely due to his catalog returning to Spotify in late 2015. LAST YEAR: 11

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26. Phish

Phish released its 13th album in 2016, but that wasn’t what moved the needle. Selling 647,000 tickets across 40 shows brought in 98.5 percent of the band’s revenue, more than enough to keep Trey Anastasio’s crew bouncing along the road. LAST YEAR: N/A

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27. Maroon 5

Maroon 5 fell from its top 10 spot in 2015 with lower touring, publishing and sales numbers, down nearly $2 million in the lattermost category. But the band’s streaming numbers saw a bump thanks to the Kendrick Lamar-featuring “Don’t Wanna Know.” LAST YEAR: 10

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28. Zac Brown Band

As they prepped a new album, 2017’s the Atlantan country crew made money on the road, between a handful of dates wrapping 2015’s Jekyll and Hyde Tour and then another 45 North American shows on the 2016 Black Out the Sun outing. LAST YEAR: N/A

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29. Black Sabbath

Black Sabbath’s touring prowess accounted for its presence here: The band grossed $33 million, bringing home over $11 million from its The End Tour. But as the name of Sabbath’s most recent outing suggests, that 2016 tour was its last. LAST YEAR: N/A

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30. Blink-182

After replacing Tom DeLonge in 2015, blink-182 staged a 2016 comeback with , its first album since 2011. The LP, plus the band’s catalog, netted nearly 544 million combined on-demand streams, spurring the band’s earnings. LAST YEAR: N/A

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31. Jennifer Lopez

Lopez’s lion’s share comes from her residency at the Axis at Planet Hollywood -- the most successful Las Vegas residency of 2016, with a gross of $34.6 million. That earned Lopez $11.8 million and a spot on this year’s list without releasing an album. LAST YEAR: N/A

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32. Elton John

While Sir Elton’s record sales were sluggish in 2016 -- his entire discography topped out around 300,000 units -- he made his strongest gain on the road. The year netted him $12.1 million, $1.5 million of that from artist and songwriter royalties. LAST YEAR: 15

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33. Dead Company

This act is all about the road, with a $32 million U.S. box-office gross translating into $11 million in take-home pay. Its catalog still produces album sales (280,000 units), but track sales (238,000 downloads) and streaming (118 million) are meager. LAST YEAR: 6

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34. David Bowie

After Bowie’s death in January 2016, his estate saw a boom in business, with nearly 1.3 million album sales and 387 million on-demand streams. His artist and songwriting royalties for the year rang in at $11.5 million, due to the estate owning his catalog. LAST YEAR: N/A

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35. Keith Urban

Urban’s eighth studio album, ,accounted for 412,000 of his 493,000 album sales in 2016, while track sales hit 2 million and combined on-demand streams were just shy of 362 million. Nearly $9 million from touring rounded out his take. LAST YEAR: N/A

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36. The Rolling Stones

The Stones almost matched their box-office booty with sales-derived royalties, up from $1.4 million in sales in 2015. The band’s first album since 2005, ,debuted at No. 4 on the Billboard 200, a record-setting 37th top 10 effort. LAST YEAR: 3

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37. Celine Dion

Dion, who lost both her husband and brother within two days in 2016, released a new album, and her Las Vegas residency at Caesars Palace boosted her live income to $10.2 million. She added $647,000 from artist royalties. LAST YEAR: 25

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38. Def Leppard

The rockers released their 11th album in 2015, though their catalog earned just 48 million on-demand streams and 14,000 digital album sales. But 172,000 physical units and a box-office gross of $29.7 million made all the difference in 2016. LAST YEAR: N/A

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39. Britney Spears

Spears’ Las Vegas residency helped push her live take-home pay to $9.5 million, while new album contributed to $1.2 million in royalties, with 529 million combined on-demand streams and 250,000 album sales total across her catalog. LAST YEAR: 22

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40. Marc Anthony

The only mostly Spanish-language act on the list, Anthony’s robust touring schedule earned him his spot. In the United States alone, he sold 221,000 tickets, grossing $28 million and keeping a hefty $9.5 million, a $3 million jump over 2015. LAST YEAR: 37

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41. Blake Shelton

Honesty appears to be the best policy for Shelton, whose 2016 LP, scored him his fifth No.1 on Top Country Albums. A total of 931,000 album sales and 582 million on-demand streams largely fed his $3.1 million in artist royalties. LAST YEAR: N/A

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42. The Weeknd

The Weeknd didn’t play a U.S. show in 2016, yet he still racked up 1.9 billion on-demand audio streams, the fourth-most of the year. He also made the top 10 in track sales (3.8 million) and terrestrial radio spins (1.2 million) as his album kicked in. LAST YEAR: 28

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43. Pearl Jam

A quarter century after the band’s debut, , Pearl Jam’s catalog racked up 562,000 track sales and 216 million streams to the tune of $1 million in royalties. Selling 351,000 tickets across just 16 U.S. shows proved the act remains in demand. LAST YEAR: N/A

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44. Journey

The ’70s hitmakers hit paydirt touring with The Doobie Brothers and selling over 500,000 tickets in the United States. Sales royalties helped: The band’s stayed on the Billboard 200 for 450 nonconsecutive weeks, the fifth-most of all time. LAST YEAR: N/A

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45. Jimmy Buffett

While his album sales totaled 200,000 units, largely from 2016’s a sluggish switch to streaming could mean storm clouds ahead for Buffett’s revenue. But a $25 million gross across 24 shows means there’s no reason to pack up the tequila yet. LAST YEAR: N/A

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46. Sia

The Australian songstress was No. 15 in publishing royalties, buoyed by her 2016 set, .“Cheap Thrills” was her first Hot 100 No. 1, but the bulk of her earnings, $5.5 million, came from her 23-date arena tour. LAST YEAR: N/A

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47. Selena Gomez

Gomez’s sales and streaming royalties remained strong last year, even though her last album, ,came out in October 2015. Her 2016 arena tour made up the bulk of her revenue, with 248,000 U.S. ticket sales generating a $17 million gross. LAST YEAR: N/A

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48. James Taylor

Taylor’s revenue is largely derived from live income -- his recorded-music earnings only account for 10 percent of his $8.3 million total. Taylor’s bottom line still got a boost from nearly 170,000 album sales, though streaming royalties were low. LAST YEAR: N/A

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49. Stevie Nicks

With streaming royalties under $100,000, Nicks’ return to the stage secured her spot on this list. Her 24 Karat Tour earned $23 million at the box office with $7.8 million in take-home pay, and she pocketed $66,000 from Fleetwood Mac. LAST YEAR: N/A

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50. Pentatonix

Even though Pentatonix grossed $12.8 million at the box office, the a cappella group needed a boost from recorded music to make the list. With $2.9 million in total artist royalties from sales, Pentatonix sold the fifth-most albums of any artist in 2016. LAST YEAR: N/A

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Billboard's Official Top 50 Money Makers List of 2016 | Billboard News
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View larger version:
Figure 3

FOG-finger 6 folds around metal ions. CD spectrum of FOG-finger 6 (694–723) in the absence of Zn (), and with 1 equivalent of Zn added (). UV and visible absorption spectra recorded during titration of Co into FOG-finger 6 (694–723). Spectra are shown at [Co]/[peptide] ratios of 0.25, 0.50, 0.75, 1.00, 1.25, and 1.50. A graph of absorbance at 310 nm [Co]/[peptide] is shown in the to demonstrate the stoichiometry of metal binding.

In order to probe the geometry of metal ion ligation and the identity of the metal-coordinating residues, we carried out UV/VIS spectrophotometry experiments in the presence of Co 2+ (a metal which is commonly used as a spectroscopic probe for zinc-binding proteins, due to its favorable electronic properties ( 42-45 ). The appearance of bands at 310 and 350 nm is indicative of cysteinate ligation ( metallic touch strap sneakers White Maison Martin Margiela QjkYb
), while the signals at 600, 650, and 720 nm show both that the metal coordination sphere is tetrahedral and that at least one nitrogen ligand is involved in the metal ligation ( Mens Chelsea Boots Marc OPolo MI12C
, Cropped Flare Trouser With Grommet Detail Blue Derek Lam kkzV1l
) (Fig. 3 B ). Taken together, the CD and UV/VIS data suggest that the FOG finger is capable of folding around zinc, with one histidine and three cysteine residues ligating the metal.

We next sought to investigate whether the integrity of the FOG zinc finger was required for its interaction with GATA-1. We prepared two FOG-finger 6 (residues 678–760) mutants in which zinc-coordinating cysteine residues were replaced by alanine, Cys 698 → Ala and Cys 719 → Ala, and assayed their ability to interact with GATA-1 (residues 200–254) in the yeast two-hybrid system. Neither mutant was able to interact with GATA-1 (Fig. Amery Maxi Dress in Mauve size L also in MSXS Cleobella czhVSU
). We also mutated the 4th cysteine to histidine, Cys 719 → His, to mimic the CCHH configuration of a classical zinc finger. This mutant was also unable to interact with GATA-1 (Fig. 4 ), suggesting that the unusual CCHC configuration is critical for this protein-protein interaction.

View larger version:
Figure 4

The integrity of the zinc-binding module in FOG is required for its interaction with GATA-1. Mutations were made in the 6th zinc finger of FOG (residues 678–760) and its ability to bind the N-finger of GATA-1 (residues 200–254) was tested by the yeast two-hybrid system as described in the legend for Fig. long lace detail dress White Ermanno Scervino SPjlX
.

To further assess the importance of metal ions, we carried out in vitro GST pull-down experiments in the presence and absence of EDTA. A 35 S-labeled in vitro -translated fragment of FOG containing finger 6 was assessed for its ability to interact with GST GATA-N-finger (residues 200–254) immobilized on agarose beads. This fragment of FOG was efficiently retained on the GATA N-finger-coated beads (Fig. Houndstooth Woolblend Wideleg Pants Black Victoria Beckham spyY6orO
, lane 2 ) but not on GST-coated beads (Fig. FOOTWEAR Lowtops amp; sneakers Bumper p6DLlgoE
, lane 4 ). When the experiment was repeated in the presence of 5 m m EDTA, the FOG fragment was no longer retained (Fig. 5 , lane 3 ). We have previously demonstrated that the GATA-1 zinc fingers bind zinc more tightly than classical fingers, and are not affected by the addition of EDTA under these conditions, 2 and we have also carried out additional controls indicating that EDTA does not affect the binding of GST GATA-1-N-finger to glutathione-coated beads (data not shown). Consequently, this particular experiment primarily addresses the importance of metal ions to the FOG finger.

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